Core Facility

Proteome Center Tuebingen (PCT) is a proteomics core facility of the University of Tuebingen and a member of the Quantitative Biology Center (www.qbic.uni-tuebingen.de).

 

We offer various workflows for qualitative and quantitative analyses of proteins, proteomes and subproteomes (e.g. interactome, phosphoproteome, acetylome, ubiquitylome). Other posttranslational modifications can also be analyzed upon request. 

 

Protein identification (e.g. from a gel spot) is routinely performed on an LTQ-Orbitrap XL mass spectrometer, whereas quantitative shot-gun (phospho)proteomics experiments are performed on an LTQ-Orbitrap Elite or a Q-Exactive HF mass spectrometer. Quantitative workflows are mainly based on stable isotope labeling of cells and tissues (SILAC) or label-free quantification. 

 

Please note that for a successful proteomics project:

  • Genome sequence or protein database of the organism of interest has to be available
  • Proteins should be delivered in µg amount as precipitated extracts or gel spots
  • Proteins/gels must not be radioactively labeled
  • Theoretical background of the project and protocols employed prior to MS measurement should be discussed with the PCT in detail in order to adapt the adequate analysis method

 

Our operational and access policies can be found here: Nutzer- und Gebührenordnung (German only).

 

For further inquiries, requests and price information please send an e-mail to pct[at]ifiz.uni-tuebingen.de.